6CC2
Crystal Structure of CDC45 from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97880 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.120, 73.810, 171.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.090 - 1.660 |
| R-factor | 0.1588 |
| Rwork | 0.157 |
| R-free | 0.19090 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.748 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((dev_3063: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.068 | 0.905 |
| Number of reflections | 57872 | |
| <I/σ(I)> | 10.5 | 1.6 |
| Completeness [%] | 96.7 | 92.1 |
| Redundancy | 5.1 | 5.1 |
| CC(1/2) | 0.601 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | sodium thiocyanate (pH 6.9), polyethyleneglycol 3,350 |
