6CBR
DnaG Primase C-terminal domain complex with SSB C-terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95368 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.723, 47.363, 51.881 |
| Unit cell angles | 63.51, 76.97, 73.81 |
Refinement procedure
| Resolution | 41.496 - 1.500 |
| R-factor | 0.1625 |
| Rwork | 0.162 |
| R-free | 0.17870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t3w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.892 |
| Data reduction software | iMOSFLM (6.2.6) |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | MOLREP (9.4.09) |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.039 | 0.275 |
| Rmeas | 0.389 | |
| Rpim | 0.039 | 0.275 |
| Number of reflections | 41299 | 5369 |
| <I/σ(I)> | 13.5 | 2.4 |
| Completeness [%] | 90.0 | 88.6 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 10% w/v PEG3000, 5 mM zinc acetate, 5 mM 1,10-phenanthroline, 100 mM sodium acetate, pH 4.6 |
