6CBD
Crystal Structure of Human Argonaute2 Bound to Three Tryptophans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979500 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.829, 116.995, 70.252 |
| Unit cell angles | 90.00, 92.64, 90.00 |
Refinement procedure
| Resolution | 35.504 - 2.203 |
| R-factor | 0.1777 |
| Rwork | 0.176 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.780 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.068 | 0.448 |
| Rpim | 0.039 | 0.261 |
| Number of reflections | 44728 | 3814 |
| <I/σ(I)> | 13.7 | 3.1 |
| Completeness [%] | 98.2 | 97 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 14% PEG 3350, 0.1 M Tris pH 8.2, 12% Isopropanol, 10 mM MgCl2, L-tryptophan to saturation |
