6CB9
Segment AALQSS from the low complexity domain of TDP-43, residues 328-333
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 4.790, 15.970, 42.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.220 - 1.100 |
R-factor | 0.1994 |
Rwork | 0.195 |
R-free | 0.24300 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.422 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 21.220 | 21.220 | 1.140 |
High resolution limit [Å] | 1.100 | 4.120 | 1.100 |
Rmerge | 0.211 | 0.141 | 0.562 |
Rmeas | 0.230 | 0.161 | 0.605 |
Number of reflections | 1432 | 39 | 119 |
<I/σ(I)> | 5.12 | 6.35 | 2.82 |
Completeness [%] | 90.7 | 100 | 72.1 |
Redundancy | 6.853 | 3.821 | 7.252 |
CC(1/2) | 0.970 | 0.973 | 0.913 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.01 M Nickel Chloride hexahydrate, 0.1 M Tris pH 8.5, 20% (w/v) PEG 2000 |