6CB3
Crystal structure of the L.Lactis YkoY riboswitch bound to cadmium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.988 |
| Spacegroup name | P 31 |
| Unit cell lengths | 73.141, 73.141, 117.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.096 - 1.891 |
| R-factor | 0.1468 |
| Rwork | 0.140 |
| R-free | 0.17370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y1i |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.656 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 56296 | |
| <I/σ(I)> | 57.98 | |
| Completeness [%] | 99.0 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 40 mM Sodium Cacodylate pH 7.0, 80 mM NaCl, 20 mM BaCl2, 5 mM CdCl2, 12 mM spermine hydrochloride, 12% 2-methyl-2,4-pentanediol |
