6CAS

Serial Femtosecond X-ray Crystal Structure of 30S ribosomal subunit from Thermus thermophilus in complex with N1MS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	402.100, 402.100, 175.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.190 - 3.500
R-factor	0.2475
Rwork	0.247
R-free	0.27730
RMSD bond length	0.003
RMSD bond angle	0.647
Data reduction software	MOSFLM
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.190	39.190
High resolution limit [Å]	3.500	3.500
Number of reflections	175871
<I/σ(I)>	2.29
Completeness [%]	100.0
Redundancy	131

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	298	17% MPD