6CAO
Structure of the ribosomal decoding complex at ambient temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 402.300, 402.300, 176.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.223 - 3.450 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.851 |
Data reduction software | CrystFEL |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.223 | 3.632 |
High resolution limit [Å] | 3.450 | 3.450 |
Number of reflections | 188443 | |
<I/σ(I)> | 5.89 | |
Completeness [%] | 100.0 | |
Redundancy | 384.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 298 | 17% MPD |