6CA6

Crystal structure of PCT64_35S, a broadly neutralizing anti-HIV antibody.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	96.776, 98.132, 100.563
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.066 - 2.430
R-factor	0.2037
Rwork	0.201
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5feh
RMSD bond length	0.006
RMSD bond angle	0.866
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.470
High resolution limit [Å]	2.430	2.430
Rpim		0.260
Number of reflections	36695
<I/σ(I)>	18.3
Completeness [%]	99.7
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.17M sodium acetate, 0.085M Tris pH 8.5, 15% glycerol and 25.5% PEG 4000