6CA4

Crystal structure of humanized D. rerio TDP2 by 14 mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	57.034, 98.260, 134.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.391 - 1.623
R-factor	0.1842
Rwork	0.183
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fpv
RMSD bond length	0.005
RMSD bond angle	0.723
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	134.720	1.650
High resolution limit [Å]	1.620	1.620
Rmerge	0.074	1.864
Rmeas	0.079	2.305
Rpim	0.030	1.587
Number of reflections	95228	8475
<I/σ(I)>	15.5
Completeness [%]	99.0	83.1
Redundancy	6.7	2.2
CC(1/2)	0.999	0.424

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG3350, Sodium Malonate