6C8T
The structure of MppP soaked with the substrate L-Arg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.792, 108.425, 196.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.055 - 2.200 |
| R-factor | 0.1475 |
| Rwork | 0.146 |
| R-free | 0.19540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dj1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.119 |
| Data reduction software | HKL-2000 ((1.11.1_2575: ???)) |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.057 | 0.193 |
| Number of reflections | 85820 | 3839 |
| <I/σ(I)> | 24.7 | 6.1 |
| Completeness [%] | 91.6 | 84.1 |
| Redundancy | 4.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 30% polyethylene glycol monomethyl ether 550, 50 mM MgCl2, and 0.1 M HEPES pH 8.0 |
