6C8D
RNA-dGMP complex with Mg ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2017-07-09 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 49.547, 49.547, 81.337 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.920 |
| R-factor | 0.22252 |
| Rwork | 0.219 |
| R-free | 0.28184 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dhc |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.987 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.140 | 0.722 |
| Rmeas | 0.153 | 0.772 |
| Rpim | 0.056 | 0.271 |
| Number of reflections | 9264 | 925 |
| <I/σ(I)> | 12.6 | 2.64 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.9 | 8 |
| CC(1/2) | 0.964 | 0.879 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 10% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.040 M Sodium cacodylate trihydrate pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.080 M Sodium chloride, 0.020 M Magnesium chloride |
