6C80
Crystal structure of a flax cytokinin oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2012-05-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953691 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 91.226, 58.929, 188.867 |
| Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
| Resolution | 49.355 - 1.780 |
| R-factor | 0.1523 |
| Rwork | 0.151 |
| R-free | 0.18370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2exr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.154 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.080 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 95714 | |
| <I/σ(I)> | 2.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.24 M tribasic ammonium citrate (pH 8.0) and 18% (w/v) PEG-3350 |
