6C7P
Crystal structure of D477G ACO/RPE65 chimera, monoclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.918401 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.431, 56.170, 136.912 |
| Unit cell angles | 90.00, 133.82, 90.00 |
Refinement procedure
| Resolution | 41.760 - 2.600 |
| R-factor | 0.22194 |
| Rwork | 0.220 |
| R-free | 0.25981 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c7k |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.107 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.066 | 0.998 |
| Number of reflections | 31744 | 5023 |
| <I/σ(I)> | 11.6 | 0.93 |
| Completeness [%] | 96.7 | 96 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 0.1 M Bis-Tris propane, pH 6 and 21% w/v sodium polyacrylate 2100 |
