6C7N
Monoclinic form of malic enzyme from sorghum at 2 angstroms resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-09-12 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 208.100, 64.260, 202.720 |
Unit cell angles | 90.00, 93.65, 90.00 |
Refinement procedure
Resolution | 29.280 - 2.000 |
R-factor | 0.18 |
Rwork | 0.180 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | TRUNCATE |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.240 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.085 | 0.585 |
Rmeas | 0.100 | 0.690 |
Rpim | 0.052 | 0.360 |
Total number of observations | 665459 | 31790 |
Number of reflections | 181154 | 8879 |
<I/σ(I)> | 9.1 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.7 | 3.6 |
CC(1/2) | 0.997 | 0.733 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2M KF, 30% (w/v) PEG3350, 2mM NADP, 2mM DTT, 40mM pyruvate 10mM MgCl2 |