6C72
Crystal structure of a tailspike protein gp49 from Acinetobacter baumannii bacteriophage Fri1, a capsular polysaccharide depolymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9796 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.462, 98.664, 148.704 |
Unit cell angles | 90.00, 91.81, 90.00 |
Refinement procedure
Resolution | 49.332 - 2.396 |
R-factor | 0.1569 |
Rwork | 0.154 |
R-free | 0.20310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | in house model |
RMSD bond length | 0.006 |
RMSD bond angle | 0.788 |
Data reduction software | XDS (March 30, 2013) |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.332 | 49.332 | 2.530 |
High resolution limit [Å] | 2.396 | 7.580 | 2.400 |
Rmerge | 0.024 | 0.405 | |
Rmeas | 0.095 | 0.037 | 0.510 |
Rpim | 0.043 | 0.017 | 0.230 |
Total number of observations | 388727 | ||
Number of reflections | 81953 | 2711 | 11224 |
<I/σ(I)> | 16 | 25.9 | 1.9 |
Completeness [%] | 98.2 | 99.1 | 92.5 |
Redundancy | 4.7 | 4.5 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 15% PEG 8000, 50 mM NaCl, 100 mM Tris HCl pH8.0 |