6C6M
IgCam3 of human MLCK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2012-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 |
| Unit cell lengths | 74.693, 74.693, 131.481 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.000 - 2.500 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.27200 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.430 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.700 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 14445 | |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 98.8 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 200mM ammonium dihydrogen phosphate, 20% PEG3350 |
