6C6K

Structural basis for preferential recognition of cap 0 RNA by a human IFIT1-IFIT3 protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2016-12-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97957
Spacegroup name	P 1
Unit cell lengths	51.957, 80.484, 88.054
Unit cell angles	79.82, 78.81, 90.04

Refinement procedure

Resolution	84.960 - 2.540
R-factor	0.1722
Rwork	0.169
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.668
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.7.17)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	84.960	50.000	2.590
High resolution limit [Å]	2.540	6.920	2.550
Rmerge	0.076	0.055	0.326
Rmeas	0.090	0.065	0.394
Rpim	0.047	0.034	0.219
Number of reflections	41031	2128	1762
<I/σ(I)>	10.1
Completeness [%]	90.9	94.1	75.6
Redundancy	3.4	3.6	2.9
CC(1/2)		0.944	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.4	293	imidazole, PEG20000