6C65
Crystal Structure of the Mango-II-A22U Fluorescent Aptamer Bound to TO1-Biotin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.722 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 36.639, 179.680, 108.313 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.381 - 2.800 |
R-factor | 0.191169345205 |
Rwork | 0.186 |
R-free | 0.23689 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.225 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.381 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.102 | 0.956 |
Number of reflections | 9216 | 1254 |
<I/σ(I)> | 3.6 | |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 11.3 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M HEPES pH 7.5, 10mM NiCl2, 2.3M Ammonium Formate, 12% glycerol |