6C5X

Crystal Structure of SOCS1 in complex with ElonginB and ElonginC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	61.122, 79.996, 132.747
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.519 - 3.105
R-factor	0.2371
Rwork	0.235
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c9w
RMSD bond length	0.002
RMSD bond angle	0.483
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.519	55.519	3.180
High resolution limit [Å]	3.100	13.860	3.100
Rmerge	0.198	0.044	1.127
Rmeas	0.222	0.050	1.265
Number of reflections	12239	171	832
<I/σ(I)>	6.88	24.52	1.43
Completeness [%]	99.1	94.5	91.5
Redundancy	4.819	3.836	4.71
CC(1/2)	0.994	0.996	0.553

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		281	18% PEG3350, 200 mM sodium fluoride, 100 mM Tris, pH 7.5