6C4Y
Cross-alpha Amyloid-like Structure alphaAmG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 125.241, 125.241, 119.026 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 125.240 - 2.500 |
R-factor | 0.23799 |
Rwork | 0.237 |
R-free | 0.25916 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.915 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 126.000 | 2.580 |
High resolution limit [Å] | 2.490 | 2.490 |
Number of reflections | 62540 | |
<I/σ(I)> | 19.2 | |
Completeness [%] | 100.0 | |
Redundancy | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M ammonium acetate, 0.1 M tri-sodium citrate pH 5.6, 30% (w/v) MPD |