6C4U
Engineered FHA with Myc-pTBD peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 70.180, 72.370, 280.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 140.180 - 2.600 |
R-factor | 0.2349 |
Rwork | 0.233 |
R-free | 0.27910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g6g |
RMSD bond length | 0.012 |
RMSD bond angle | 1.704 |
Data reduction software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 140.180 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmeas | 0.069 | |
Number of reflections | 47879 | |
<I/σ(I)> | 16.08 | 1.17 |
Completeness [%] | 98.4 | 90.4 |
Redundancy | 6.96 | 5.78 |
CC(1/2) | 0.990 | 0.867 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1.4 M Sodium Malonate |