6C35
Mycobacterium smegmatis flap endonuclease mutant D148N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.439, 40.085, 68.419 |
Unit cell angles | 90.00, 109.11, 90.00 |
Refinement procedure
Resolution | 38.164 - 1.803 |
R-factor | 0.1596 |
Rwork | 0.157 |
R-free | 0.19310 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.903 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rpim | 0.031 | 0.133 |
Number of reflections | 55715 | |
<I/σ(I)> | 22.2 | 3 |
Completeness [%] | 98.0 | 87.2 |
Redundancy | 3.9 | 2.8 |
CC(1/2) | 0.995 | 0.915 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M LiCl, 20% PEG3350 |