6C1B
FGFR1 kinase complex with inhibitor SN37118
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-04 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 213.514, 52.193, 65.795 |
| Unit cell angles | 90.00, 107.03, 90.00 |
Refinement procedure
| Resolution | 19.450 - 2.000 |
| R-factor | 0.22213 |
| Rwork | 0.220 |
| R-free | 0.25504 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wun |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.655 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.450 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | |
| Rpim | 0.027 | 0.453 |
| Number of reflections | 46401 | 3302 |
| <I/σ(I)> | 18.9 | 1.9 |
| Completeness [%] | 98.6 | 96 |
| Redundancy | 7.6 | 7.5 |
| CC(1/2) | 1.000 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% MPEG 5000, 0.1 M sodium cacodylate pH 7.5, 0.2 M ammonium sulfate |
