6C1A
MBD2 in complex with methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.533, 39.875, 105.151 |
| Unit cell angles | 83.97, 85.70, 62.75 |
Refinement procedure
| Resolution | 34.840 - 2.050 |
| R-factor | 0.2319 |
| Rwork | 0.231 |
| R-free | 0.26520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | currently unpublished model |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.517 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.340 | 35.340 | 2.110 |
| High resolution limit [Å] | 2.050 | 8.940 | 2.050 |
| Rmerge | 0.067 | 0.022 | 0.663 |
| Rmeas | 0.090 | 0.030 | 0.895 |
| Rpim | 0.060 | 0.020 | 0.597 |
| Total number of observations | 69361 | 803 | 5524 |
| Number of reflections | 31675 | 366 | 2520 |
| <I/σ(I)> | 9.6 | 30.4 | 1.4 |
| Completeness [%] | 96.2 | 92 | 96.8 |
| Redundancy | 2.2 | 2.2 | 2.2 |
| CC(1/2) | 0.996 | 0.998 | 0.495 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 20% PEG-3350, 0.2 M ammonium chloride |
