6C0Z

Crystal structure of Efga from Methylobacterium extorquens in complex with formaldehyde

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97934
Spacegroup name	P 21 2 21
Unit cell lengths	72.059, 72.063, 104.555
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.315 - 1.830
R-factor	0.2242
Rwork	0.222
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a2l
RMSD bond length	0.010
RMSD bond angle	0.919
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.860
High resolution limit [Å]	1.830	4.970	1.830
Rmerge	0.119	0.094	0.545
Total number of observations	557370
Number of reflections	48026	2645	2293
<I/σ(I)>	7.4
Completeness [%]	98.4	99.4	96.3
Redundancy	11.6	11.8	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283.15	0.2 M KNO3, 2.2 M AmSO4, 16.6 mM Formaldehyde