6C01

Human ectonucleotide pyrophosphatase / phosphodiesterase 3 (ENPP3, NPP3, CD203c)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-17
Detector	RAYONIX MX-300
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	96.184, 133.640, 167.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.272 - 2.300
R-factor	0.1751
Rwork	0.174
R-free	0.21380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b56
RMSD bond length	0.005
RMSD bond angle	0.705
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	96743
<I/σ(I)>	13.1
Completeness [%]	100.0
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1M buffer PCB (QIAGEN PACT suite), 25% PEG 1500