6BZY

Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the 22D11 broadly neutralizing antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	46.429, 79.850, 126.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.137 - 1.600
R-factor	0.1898
Rwork	0.188
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bzu
RMSD bond length	0.006
RMSD bond angle	0.860
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	62992
<I/σ(I)>	41.8
Completeness [%]	99.7
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% (w/v) PEG 8000, 0.1 M sodium chloride, 0.1 M CAPS pH 10.5