6BZW
Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the GL precursor of the broadly neutralizing antibody AP33
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.356, 84.709, 192.919 |
Unit cell angles | 90.00, 89.96, 90.00 |
Refinement procedure
Resolution | 49.155 - 2.200 |
R-factor | 0.2127 |
Rwork | 0.192 |
R-free | 0.23060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g6a |
RMSD bond length | 0.002 |
RMSD bond angle | 0.629 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rpim | 0.089 | 0.417 |
Number of reflections | 93841 | |
<I/σ(I)> | 11.85 | |
Completeness [%] | 95.4 | |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG 4000, 20% (w/v) glycerol, 0.16 M ammonium-sulfate, 0.08 M acetate pH 4.6 |