6BZM
GFGNFGTS from low-complexity/FG repeat domain of Nup98, residues 116-123
Experimental procedure
Source type | ELECTRON MICROSCOPE |
Source details | TECNAI F20 TEM |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2017-03-23 |
Detector | TVIPS F416 CMOS CAMERA |
Wavelength(s) | 0.0251 |
Spacegroup name | P 1 |
Unit cell lengths | 4.790, 18.240, 26.440 |
Unit cell angles | 93.14, 92.73, 97.15 |
Refinement procedure
Resolution | 26.360 - 0.900 |
R-factor | 0.23 |
Rwork | 0.226 |
R-free | 0.26400 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.014 |
RMSD bond angle | 1.160 |
Data scaling software | XSCALE |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 26.360 | 26.360 | 0.920 |
High resolution limit [Å] | 0.900 | 4.030 | 0.900 |
Rmerge | 0.289 | 0.255 | 0.588 |
Rmeas | 0.313 | 0.270 | 0.717 |
Total number of observations | 31674 | ||
Number of reflections | 5800 | 63 | 247 |
<I/σ(I)> | 3.51 | 8.13 | 1.12 |
Completeness [%] | 88.0 | 87.5 | 47.8 |
Redundancy | 5.461 | 6.714 | 2.798 |
CC(1/2) | 0.976 | 0.972 | 0.546 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 0.1 M CHES, pH 9.5, 10% ethanol |