6BZF
Structure of S. cerevisiae Zip2:Spo16 complex, C2 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 169.402, 63.589, 199.043 |
Unit cell angles | 90.00, 90.60, 90.00 |
Refinement procedure
Resolution | 57.093 - 2.286 |
R-factor | 0.1984 |
Rwork | 0.196 |
R-free | 0.24980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bzg |
RMSD bond length | 0.008 |
RMSD bond angle | 0.873 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.330 |
High resolution limit [Å] | 2.286 | 2.286 |
Number of reflections | 95052 | 10085 |
<I/σ(I)> | 11.1 | |
Completeness [%] | 98.6 | |
Redundancy | 3.5 | |
CC(1/2) | 0.997 | 0.328 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.6 | 293 | 1.4 M Na-K phosphate pH 6.6 |