6BZ7

Thermus thermophilus 70S containing 16S G299A point mutation and near-cognate ASL Leucine in A site.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	210.696, 446.140, 616.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	145.572 - 3.680
R-factor	0.2299
Rwork	0.228
R-free	0.27430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v97
RMSD bond length	0.003
RMSD bond angle	0.700
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	145.572	145.572	3.730
High resolution limit [Å]	3.640	16.280	3.640
Rmerge	0.298	0.057	1.169
Rmeas	0.323	0.061	1.302
Total number of observations	4363101
Number of reflections	638848	7363	46922
<I/σ(I)>	6.23	25.04	1.38
Completeness [%]	99.3	95	99.3
Redundancy	6.83	7.593	5.225
CC(1/2)	0.991	0.995	0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	3.8% PEG 20k, 3.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC