6BYP

Structure of PL24 family Polysaccharide lyase-LOR107

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-11
Detector	RAYONIX MX300HE
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	83.551, 121.263, 123.811
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.072 - 1.900
R-factor	0.176
Rwork	0.175
R-free	0.20200
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.802
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CRANK2
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.072	1.950
High resolution limit [Å]	1.890	1.900
Number of reflections	99600
<I/σ(I)>	16.56
Completeness [%]	99.9
Redundancy	15
CC(1/2)	0.999	0.652

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	23% PEG8K, 0.085M MES pH6.5, 0.17M Ammonium Sulfate, 15% Glycerol