6BY9
Crystal structure of EHMT1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 53.681, 53.681, 204.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.490 - 2.300 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.490 | 46.490 | 2.380 |
| High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
| Rmerge | 0.061 | 0.037 | 1.251 |
| Rmeas | 0.066 | 0.040 | 1.336 |
| Rpim | 0.024 | 0.016 | 0.463 |
| Total number of observations | 127072 | 2100 | 12370 |
| Number of reflections | 16043 | 345 | 1531 |
| <I/σ(I)> | 15.6 | 42.5 | 1.9 |
| Completeness [%] | 99.8 | 99.7 | 99.3 |
| Redundancy | 7.9 | 6.1 | 8.1 |
| CC(1/2) | 0.998 | 0.997 | 0.938 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M BIS-TRIS pH6.5,3.0M NaCl |
