6BXW

Crystal structure of Toxoplasma gondii Mitochondrial Association Factor 1 B (MAF1B) in complex with ADPribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	45.470, 62.360, 89.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.556 - 1.650
R-factor	0.1969
Rwork	0.196
R-free	0.21910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bxr
RMSD bond length	0.004
RMSD bond angle	0.856
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.556	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.094	0.553
Number of reflections	29065
<I/σ(I)>	7.6	2.2
Completeness [%]	93.4	97.4
Redundancy	3.9	3.8
CC(1/2)	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2 M ammonium sulfate, 20% PEG3350