6BXR
Crystal structure of Toxoplasma gondii Mitochondrial Association Factor 1 B (MAF1B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-29 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9195 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.090, 62.110, 89.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.605 - 1.600 |
| R-factor | 0.1751 |
| Rwork | 0.173 |
| R-free | 0.21320 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.819 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.605 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.475 | |
| Number of reflections | 33786 | |
| <I/σ(I)> | 12.7 | 3.5 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 8.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 0.2 M ammonium sulfate, 20% PEG3350 |
