6BXI

X-ray crystal structure of NDR1 kinase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	117.191, 117.967, 95.044
Unit cell angles	90.00, 124.88, 90.00

Refinement procedure

Resolution	48.071 - 2.200
R-factor	0.1726
Rwork	0.171
R-free	0.21230
RMSD bond length	0.008
RMSD bond angle	1.184
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1951)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.071	2.450
High resolution limit [Å]	2.200	2.200
Number of reflections	220717
<I/σ(I)>	8.3
Completeness [%]	98.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	4 M ammonium acetate, 0.1 M Bis-Tris propane pH 7