6BX7

Crystal Structure of Human Protocadherin-1 EC1-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97921
Spacegroup name	P 61 2 2
Unit cell lengths	146.981, 146.981, 147.686
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.260 - 2.850
R-factor	0.22213
Rwork	0.221
R-free	0.24336
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dzv
RMSD bond length	0.010
RMSD bond angle	1.484
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.156	1.627
Rmeas	0.164	1.710
Rpim	0.050	0.521
Number of reflections	22567	1103
<I/σ(I)>	18.3	1.92
Completeness [%]	100.0	100
Redundancy	10.7	10.6
CC(1/2)	0.998	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.2 M Calcium Chloride, 0.1 M HEPES Sodium Salt pH 7.5, 15% PEG 400 + 15% Glycerol