6BVZ

Importin alpha 3 in cargo free state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	47.118, 53.229, 91.309
Unit cell angles	90.00, 92.86, 90.00

Refinement procedure

Resolution	19.814 - 2.300
R-factor	0.251
Rwork	0.250
R-free	0.26850
RMSD bond length	0.005
RMSD bond angle	1.029
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.814	2.380
High resolution limit [Å]	2.300	2.300
Rpim	0.086	0.304
Number of reflections	19290	1942
<I/σ(I)>	11.3	1.8
Completeness [%]	95.0	98.2
Redundancy	5.5	6.2
CC(1/2)	0.977	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M sodium nitrate, 0.1 M bis-tris propane pH 6.5, 25% w/v PEG3350