6BVT
Importin alpha 1 in cargo free state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.516, 90.007, 100.686 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.540 - 2.500 |
R-factor | 0.193 |
Rwork | 0.192 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fc8 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.586 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.540 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rpim | 0.061 | 0.365 |
Number of reflections | 24805 | 2822 |
<I/σ(I)> | 10.2 | 2.9 |
Completeness [%] | 98.6 | 100 |
Redundancy | 6.2 | 6.2 |
CC(1/2) | 0.989 | 0.751 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.01M DTT, 0.1M sodium HEPES, 1M Ammonium Sulfate |