6BUS
Extended E2 DNA-binding domain of the Bovine Papillomavirus-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.39 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 55.203, 55.203, 203.091 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.790 - 1.900 |
| R-factor | 0.2161 |
| Rwork | 0.214 |
| R-free | 0.26050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bop |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.763 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.800 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | |
| Rpim | 0.045 | 0.419 |
| Number of reflections | 29354 | 1816 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 5.1 | |
| CC(1/2) | 0.998 | 0.543 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | sodium citrate, PEG 4000 and ammonium acetate |
