6BTX
Structure of a bacterial metal transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-18 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.072, 54.109, 72.442 |
| Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
| Resolution | 42.904 - 3.200 |
| R-factor | 0.2264 |
| Rwork | 0.223 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.521 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.904 | 3.314 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 6989 | |
| <I/σ(I)> | 6.29 | |
| Completeness [%] | 96.2 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100 mM Tris pH 8.0, 100 mM Na-acetate, 70 mM CaCl2, and 20% PEG500DME |
