6BTC

SCCmec type IV LP1413 - nucleic acids binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 61 2 2
Unit cell lengths	97.033, 97.033, 127.794
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.517 - 2.177
R-factor	0.223309918529
Rwork	0.220
R-free	0.25119
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.661
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.517	2.236
High resolution limit [Å]	2.177	2.177
Number of reflections	18476
<I/σ(I)>	27.3
Completeness [%]	96.8
Redundancy	27.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287.15	100mM NaCitrate, pH6, 10mM MgCl2-1.8M Li2SO4-14% Glycerol-5mM DTT