6BSZ

Human mGlu8 Receptor complexed with glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-05
Detector	RAYONIX MX225HE
Wavelength(s)	0.9793
Spacegroup name	I 2 2 2
Unit cell lengths	112.539, 166.623, 198.661
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 2.650
R-factor	0.2516
Rwork	0.250
R-free	0.27450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	rat mGlu7 (rmGlu) ATD (PDB: 2D4Z)
RMSD bond length	0.005
RMSD bond angle	0.790
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.940	2.790
High resolution limit [Å]	2.650	2.650
Number of reflections	51038
<I/σ(I)>	8.8
Completeness [%]	98.9
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	100 mM sodium citrate pH 6, 1.6 M ammonium sulfate, 0.2 M sodium potassium tartrate, and 5 mM L-glutamate.