6BSM
BMP1 complexed with a reverse hydroxamate - compound 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-05-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 101.789, 101.789, 101.789 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.500 - 2.330 |
| R-factor | 0.1933 |
| Rwork | 0.192 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.361 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 10.000 | 2.410 |
| High resolution limit [Å] | 2.330 | 5.020 | 2.330 |
| Rmerge | 0.068 | 0.044 | 0.576 |
| Total number of observations | 140111 | ||
| Number of reflections | 15311 | 1625 | 1502 |
| <I/σ(I)> | 12.8 | ||
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 9.2 | 8.7 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 5% PEG3350, 200 mM KSCN, 10mM spermadine |
