6BSI
Structure of HIV-1 RT complexed with an RNA/DNA hybrid containing the polypurine-tract sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 163.850, 163.850, 129.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.299 - 3.250 |
| R-factor | 0.1951 |
| Rwork | 0.193 |
| R-free | 0.24080 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.165 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 3.310 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 31873 | 3135 |
| <I/σ(I)> | 15.22 | 1.96 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 9.4 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | Sodium citrate pH 5.2, CaCl2, PEG400 |
