6BRL

Crystal structure of a glutamate tRNA ligase from Elizabethkingia meningosepticum CCUG26117 in complex with its amino acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-16
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	43.260, 111.890, 130.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.854 - 2.000
R-factor	0.1705
Rwork	0.168
R-free	0.21390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gri 2ja2 and 2WMX
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX ((dev_2947))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.854	35.854	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.063	0.029	0.572
Rmeas	0.069	0.032	0.624
Number of reflections	43563	552	3169
<I/σ(I)>	17.75	40.94	3.21
Completeness [%]	99.8	95.7	99.9
Redundancy	6.092	4.971	6.175
CC(1/2)	0.999	0.999	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	290	MCSG1 E10 (295949e10): 200mM Ammonium tartarate dibasic, 20% (w/v) PEG3350: ElmeA.01348.a.B1.PW38364 16.23 mg/mL, direct cryo: xxk4-10