6BRI
RHCC with unreduced and reduced Mercury complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.8472 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.823, 108.823, 70.388 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.687 - 3.266 |
| R-factor | 0.2836 |
| Rwork | 0.282 |
| R-free | 0.29980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ybk |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.505 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.690 | 3.350 |
| High resolution limit [Å] | 3.266 | 3.266 |
| Rmerge | 0.071 | 0.780 |
| Number of reflections | 7550 | |
| <I/σ(I)> | 3.8 | 2.8 |
| Completeness [%] | 98.7 | 95.1 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 277.15 | 2M Ammonium Sulfate 200 mM Tris pH 7.9 0.7mM K2HgI4 soaked in 4 days prior to collection |
