6BPH
Crystal structure of the chromodomain of RBBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-18 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 41.493, 41.493, 81.349 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.900 - 1.850 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2lcc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.970 | 36.970 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
| Rmerge | 0.072 | 0.026 | 1.077 |
| Rmeas | 0.075 | 0.027 | 1.119 |
| Rpim | 0.020 | 0.009 | 0.300 |
| Total number of observations | 92703 | 728 | 5174 |
| Number of reflections | 7057 | ||
| <I/σ(I)> | 26.2 | 69.7 | 3.3 |
| Completeness [%] | 99.6 | 99.8 | 98.8 |
| Redundancy | 13.1 | 8.7 | 13.3 |
| CC(1/2) | 1.000 | 1.000 | 0.919 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% PEG 3350, 0.2M magnesium chloride, 0.1M HEPES, pH 7.5 |
