6BPD

Plasmodium vivax invasion blocking monoclonal antibody 10B12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	118.540, 42.660, 105.034
Unit cell angles	90.00, 122.03, 90.00

Refinement procedure

Resolution	44.525 - 2.320
R-factor	0.2136
Rwork	0.211
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5en2
RMSD bond length	0.006
RMSD bond angle	0.994
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	Aimless (0.2.7)
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.250	2.400
High resolution limit [Å]	2.320	2.320
Number of reflections	19567	1882
<I/σ(I)>	8.5	1.6
Completeness [%]	99.5	98.8
Redundancy	3.5	3.5
CC(1/2)	0.992	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	20% (w/v) PEG 4,000, 0.1 M sodium citrate/citric acid, 20% (v/v) 2-propanol