6BPA

Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to monoclonal antibody 3E9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 2 21 21
Unit cell lengths	59.070, 177.100, 178.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.051 - 2.530
R-factor	0.1947
Rwork	0.194
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w53 5en2
RMSD bond length	0.004
RMSD bond angle	0.632
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	Aimless (0.2.7)
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.540	2.590
High resolution limit [Å]	2.530	2.530
Number of reflections	63529	4411
<I/σ(I)>	9.9	1.9
Completeness [%]	99.8	99.3
Redundancy	6.7	6.4
CC(1/2)	0.993	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	0.2 M KBr, 15% (w/v) PEG 4,000 and 0.05 M sodium citrate/citric acid